The effect of the Eminium regelii extract on cellular immunity

Абстракт

The article presents the results of a study on the effects of the wild medicinal plant Eminium regeln Vved. extract to the body of intact and subjected to emotional stress animals on the background of a long-term low dose of gamma-radiation. The reaction of the animal organism to the effects of radiation, emotional stress and the introduction of the E. regeln extract were evaluated by the total content of leukocytes, absolute and relative content of lymphocytes (including CD3+, CD4+, CD8+), immunoregulatory index (IRI) and leukocyte migration inhibition reaction (LMIR). The experiments were performed on adult mongrel white rats of both sexes. At the early stage of the general adaptation syndrome (GAS) the injection of E. regeln extract caused a decrease in the total number of lymphocytes, an increase in the number ofCD4+ lymphocytes and leukocytes lymphokinproduction abilities. Late stage of GAS was indicated by increased CD3+ and CD4+ lymphocytes, and increased values of the immunoregulatory index.

References:

Artemova A. G. 1973. The phenomenon of inhibition of white blood cells in guinea pigs with delayed-type hypersensitivity to the foreign tissue agent. - Bjulleten' jeksperimental'noj biologii meditsiny 76 (10): 67-71 (In Russian).

Buldakov L. A. & Kalistratova V. S. 2005. Radiation effects on the body - the positive effects. Moskva: Inform-Atom. ISBN: 5-89107-042-1 (In Russian).

Cho K., Jwa M.-S., Moon H.-Na, Hur S.-P., Kim D. & Yeo I.-K. 2016. Hormetic effect of 60Co gamma radiation on tolerance to salinity and temperature stress in Haliotis discus discus. - Aquaculture 451: 473-479.

El-Halim A., Kassem M. A. & Mohamed S. S. M. 2015. Impacts on the third line of defense specialized against microbial infection as a result of exposure to gamma-radiation. - Pak. J. Pharm. Sci. 28 (5): 1839-1843.

Gapeyev A. B., Aripovsky A. V. & Kulagina T. P. 2015. Modifying effects of low-intensity extremely high-frequency electromagnetic radiation on content and composition of fatty acids in thymus of mice exposed to X-rays. - Int. J. Radiat. Biol. 91 (3): 277-285.

Garib F. Yu., Garib V. Yu. & Rizopulu A. P. 1995. A method for determining a subpopulation of lymphocytes. Patent of the Republic of Uzbekistan 1111 №2426 Riz (In Russian).

Hekim N., Cetin Z., Nkitaki Z., Cort A. & Saygili E. I. 2015. Radiation triggering immune response and inflammation. - Cancer Lett. 368 (2): 156-163.

Islamian J. P. & Mehrali H. 2015. Lycopene as a carotenoid provides radioprotectant and antioxidant effects by quenching radiation-induced free radicals singlet oxygen: an overview. - Cell J. 16 (4): 386-391.

Ivanov A. A., Dorozhkina O. V., Lyakhova K. N. & Bulynin T. M. 2015. Early radiobiological effects in mice after y-irradiation in small doses. - Aviakosmicheskaya ekologicheskaya meditsina 49 (3): 12-18 (In Russian).

Luan Y. C., Shieh M. C., Chen S. T., Kung H. T., Soong K. L., Yeh Y. C., Chou T. S., Fang W. C., Yao S. L., Pong C. J., Mong S. H., Wu J. T., Wu J. M., Jeh H. J., Cher W. L., Deng W. P., Wu M. F., Shen M. L. & Sun C. P. 2006. Re-examining the health effects of radiation and its protection. - Int. J. Low Radiat. 3 (1): 27-44. doi: 10.1504/ijlr.2006.010006

Luckey T. D. 2007. Radiation prevents much cancer. - Int. J. Low Radiat. 4: 336-344.

Minana E., Roldan M., Chivato T., Martinez T. & Fuente T. 2015. Quantification of the chromosomal radiation damage induced by labelling of leukocytes with [18F] FDG. - Nucl. Med. Biol. 42 (9): 720-723.

Monfared A. S., Jatali F., Sedaghat S., Mansoorizade E., Jarrahi A., Harahmadi M. & Samavat H. 2006. High natural background radiation areas in Ramsar, Iran: can inhabitants feel safe? - Int. J. Low Radiat. 3 (2/3): 171-177.

Montsevichyute-Eringene E. V. 1961. Simplified mathematical and statistical methods in medical research. - Patologicheskaja fiziologija jeksperimen tal'naja terapija 1: 71-76 (In Russian).

Nair R. R., Rajan B., Akiba S., Jayalekshmi P., Nair M. K., Gangadharan P., Koga T., Morishima H., Nakamura S. & Sugahara T. 2009. Background radiation and cancer incidence in Kerala, India-Karanagappally cohort study. - Health Phys. 96 (1): 55-66.

Nomura T., Sakai K., Ogata H. & Magae J. 2013. Prolongation of life span in the accelerated aging klotho mouse model, by low-dose-rate continuous irradiation. - Radiat. Res. 179 (6): 717-724.

Oujifard A., Amiri R., Shahhosseini G., Davoodi R. & Moghaddam J. A. 2015. Effect of gamma radiation on the growth, survival, hematology and histological parameters of rainbow trout (Oncorhynchus mykiss) larvae. - Aquat. Toxicol. 165: 259-265.

Silybayeva B. M., Tazabayeva K. A. & Zharykbasova K. S. 2014. Biological specifics and chemical composition of medicinal plant Eminhim regeln Vved. - Global J. Pharmacol. 8 (3): 432-436. http://www.idosi.org/gjp/8(3)14/19.pdf

Vivek Kumar P. R., Seshadri M., Jaikrishan G. & Das B. 2015. Effect of chronic low dose natural radiation in human peripheral blood mononuclear cells: Evaluation of DNA damage and repair using the alkaline comet assay. - Mutat Res. 775: 59-65.

Zhetpisbayev B. A., Nurmuhambetov Zh. N. & Shabdarbayeva D. M. 1999. Method for reproducing a stress state in small laboratory animals. Patent of the Republic of Kazakhstan A.C. №2590 (In Russian).

Zharykbasova K. S., Tazabayeva K. A., Shaken T. E. & Chulenbayeva L. E. 2015. The inhibitory effect of some plant components on the growth of cancer HCT-15 cells. - Vestnik gosudarstvennogo universiteta imeni Shakarima goroda Semej 4 (72): 188-192 (In Russian).